MyBioSource.com's FAM65B Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, and Western Blot. The FAM65B Antibody was generated using RIPOR2 as the antigen and it reacts with Human, Mouse, and Rat.
Description
Function: Acts as an inhibitor of the small GTPase RHOA and plays several roles in the regulation of myoblast and hair cell differentiation, lymphocyte T proliferation and neutrophil polarization (PubMed:17150207, PubMed:24687993, PubMed:23241886, PubMed:24958875, PubMed:25588844, PubMed:27556504). Inhibits chemokine-induced T lymphocyte responses, such as cell adhesion, polarization and migration (PubMed:23241886). Involved also in the regulation of neutrophil polarization, chemotaxis and adhesion (By similarity). Required for normal development of inner and outer hair cell stereocilia within the cochlea of the inner ear (By similarity). Plays a role for maintaining the structural organization of the basal domain of stereocilia (By similarity). Involved in mechanosensory hair cell function (By similarity). Required for normal hearing (PubMed:24958875).
Subunit Structure: Homooligomer; homooligomerization is regulated by RHOC and leads to the formation of concatemers through the association of N-and C-termini (By similarity). Interacts with 14-3-3 proteins; these interactions occur during myogenic cell differentiation (PubMed:24687993). Interacts with HDAC6; this interaction occurs during early myogenic differentiation and prevents HDAC6 to deacetylate tubulin (PubMed:24687993). Interacts with DYSF; this interaction occurs during early myogenic differentiation (PubMed:24687993). Interacts with MYOF (PubMed:24687993). Interacts with RHOC (By similarity). Isoform 1 and isoform 2 interact (via active GTP-or inactive GDP-bound forms) with RHOA; these interactions are direct, block the loading of GTP to RHOA and decrease upon chemokine CCL19 stimulation in primary T lymphocytes (PubMed:23241886, PubMed:25588844). Isoform 2 interacts (phosphorylated form) with HDAC6; this interaction induces T cell proliferation arrest (PubMed:27556504). Isoform 2 interacts (phosphorylated form) with 14-3-3 proteins; these interactions induces T cell proliferation arrest (PubMed:27556504). Isoform 2 interacts with 14-3-3 proteins (PubMed:25588844). Isoform 2 interacts (via phosphorylated form) with YWHAB; this interaction occurs in a chemokine-dependent manner and does not compete for binding of RIPOR2 with RHOA nor blocks inhibition of RIPOR2-mediated RHOA activity (PubMed:25588844). Isoform 2 interacts with YWHAE (PubMed:25588844). Isoform 2 interacts with YWHAQ (PubMed:25588844).
Post-translational Modifications: Phosphorylated. Isoform 2 is phosphorylated in T cells (PubMed:27556504). Chemokine-induced phosphorylation of isoform 2 in neutrophils occurs in a PKC-and AKT-dependent manner, resulting in RIPOR2 interaction with YWHAB and stabilization (PubMed:25588844). Isoform 2 is phosphorylated by PKCA, AKT1 and MAPKAPK1A; in vitro (PubMed:25588844). Acetylated during myogenic differentiation.
Similarity: Belongs to the RIPOR family